Green spermatozoids search reasons of man sterility
Oxford scientists have developed a new technology for spermatozoid activity investigation which will help to understand the reasons of man sterility.
An expert group under leadership of John Parrington excreted a special gene from medusa organism which is responsible for production of green fluoresce protein. Using this gene, scientists managed to color spermatozoids in hamsters? testicles in green. A hamster was chosen for experiments on purpose because his sperm is similar to humans and these animals are considered as ideal models for investigation of problems during spermatozoid production.
Many cells of human organism can be cultivated and genetically modifies in laboratory conditions. But spermatozoids are exclusion. The main reasons are their small sizes and unusual form and also short period of life outside human?s body. New technology helps to analyze various aspects of gene functions in sperm. Scientists want to concentrate their efforts on investigation of spermatozoid penetration in ovule. Using medusa protein it will be possible to color in green genes responsible for ovulation and observe their activity. A green fluorescence can not be seen by human eye but is noticeable under microscope and light of certain wavelength. This technique will help to find new methods of genetically modified animal?s formation for all sorts of investigations.